Hypoxia in fat cells, what microarray said..
Finally released… After several speculations and controversies about how hypoxia on adipose tissue would affect the behaviour of this tissue, some research group finally revealed some interesting findings. In this blog i am going to comment about two published article on this very interesting field.
Firstly, last year PlosOne published an article written by groups of research intitude from several countries in Europe and US. Using microarray analysis, they found that hypoxia is able to induce certain reactions in SGBS cell. Basically, SGBS cell is a fat cell that has ability to differentiate. This is good so we can induce the differentitation ourself. Placed in hypoxic environtment (1% oxygen) for 3, 6 and 16 hours, the SGBS cell gave such a response in several pathway.
Analyzed by microarray, the authors, Geiger et al. found that genes regulation several processes including glycolysis (10 genes), response to hypoxia (16 genes), regulation of cellular component movement (17 genes), response to nutrient level (16 genes), regulation of cell migration (13 genes) and transcription regulator activity (60 genes). Importantly, those genes are related to regulation of obesity, type 2 diabetes, and metabolic syndrome. Thus, the study could give benefit impact on the ongoing study of obesity and hypoxia.
The other study using human adipose cells also been published earlier this year. Using the same method, microarray, Mazzatti et al. also showed influence of hypoxia on adipocyte genes. Tweleve days after differentiation, adipocytes were exposed to hypoxia environtment. They found 5 top pathways that are regulated by almost no oxygen condition, those including glucose uptake, lipid oxidation, hypoxia, lipolysis and gluconeogenesis. This study support that lipolysis process is altered during hypoxia. Hypoxia also related to reduced in adipogenesis since signal expression of PGC-1alpha is down regulated. Network pathway of the genes was also shown to be altered including PDK4, IDH3A, SLC25A20, cytochrome C, UCP2, adiponectin and cpt2.
Using data from both studies, the role of hypoxia on obesity is going to be an interesting area to be studied.
Geiger K et al. Identification of hypoxia-induced genes in Human SGBS adipocytes by microarry analysis. Plosone, 2011, 6(10), e26465
Mazzatti et al. A microarray analysis of the hypoxia-induced modulation of gene expression in human adipocytes. Archives of physiology and biochemistry, 2012, 118, 112-120.